Applications of Analytical Ultracentrifuge to Molecular Biology and Pharmaceutical Science

The analytical ultracentrifuge spins again

Uppsala University, BiomedicalCenter, p. O. Box 574, 5-751 23 Uppsala, Sweden. Goran Pettersson /b at the Department of Biochemistry, Uppsala University, Biomedical Center, P. O. Box 576, 5-751 23 Uppsala, Sweden. Ingrid Marle is at Pharmaceutical and Analytical R&D, Astra Pain ControlAB, S-151 85 Sodertdlje, Sweden. Jerry Stdlberg is at the Department of Molecular Biology, Uppsala University, ...

Applications of Combinatorics to Molecular Biology

Ivis detector for the analytical ultracentrifuge

A multiwavelength UV/vis detector for the analytical ultracentrifuge (MWL-AUC) has been developed recently. In this work, [3-carotene-gelatin composite particles are investigated with MWL-AUC. Band centrifugation with a Vinograd cell is used to ensure maximum sample separation. Spectral changes of the system are observed in dependence of the sedimentation coefficient and are attributed to a pre...

Self-associating Systems in the Analytical Ultracentrifuge

Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell

The analytical ultracentrifuge (AUC) is a powerful biophysical tool that allows us to record macromolecular sedimentation profiles during high speed centrifugation. When properly planned and executed, an AUC sedimentation velocity or sedimentation equilibrium experiment can reveal a great deal about a protein in regards to size and shape, sample purity, sedimentation coefficient, oligomerizatio...